Fermenter must be thoroughly disinfected in the work, because the design structure of fermenter is relatively complex. It is difficult for us to thoroughly and comprehensively disinfect the fermenter, so in order to ensure the quality of the work, the following are the detailed steps of the fermenter disinfection and sterilization method.

1. Install electrodes in the fermenter. Before sterilization, check whether the appearance of each interface and pipe is normal and whether the steam pipe is firm. Each valve should be checked for complete closure by two persons. At least two people should be present when the fermenter is sterilized. One operated the various valves and the other observed the reported parameters. After confirming that the valves of the fermenter are closed, open the water inlet valve of the steam generator, start the steam generator, and the steam generator starts to automatically water. At this time, the drainage valve under the steam generator should be opened, and the drainage valve should be closed after the rust is discharged as far as possible. Observe the water level in the steam generator to prevent accidents.

2. The fermenter fully opens the jacketed outlet valve, slowly opens the steam generator main valve, slowly opens part of the jacketed inlet valve, and starts to insert water vapor into the jacketed. At first, the steam pressure may be unstable and the gauge pointer wobbles sharply. The jacket intake valve can be properly closed, and then the steam valve of the sampling valve, the steam valve of the sampling valve, the steam valve of the sampling valve, the steam valve of the sampling valve and the steam valve of the bottom valve, to sterilize the sampling valve and the bottom valve, and to discharge the steam of unstable pressure. When the water vapor pressure does not change drastically, close the bottom valve outlet valve in turn.

3. When the temperature in the fermenter rises gradually to 90°, the stirring motor stops rotating. At this point, open the tank main intake valve, open the tank outlet valve, and open the following valves in turn: Open the outlet gas regulating valve, slowly open the tank inlet steam valve, the steam into the tank, air filter surface pressure begins to rise, more than 0.1MP open the condensate valve drain, continue to open the large tank into the steam valve, remove the tank air, tank pressure slowly rise, adjust the outlet gas regulating valve, to meet the temperature pressure required for sterilization, sterilization temperature began.

4. Adjust jacket inlet valve, tank inlet steam valve and outlet regulating valve to maintain temperature and tank pressure. When sterilization time arrives, close the opened intake valve, close the steam main valve, completely open the exhaust valve in the tank, release the pressure in the tank, and close the power supply of the steam generator. Open the air compressor, wait until the tank pressure drops to 0.05MP, slowly open the intake regulating valve, and maintain the positive pressure in the tank.

5, in the fermentation tank sterilization process, the operator should wear pants, wear protective gloves, to prevent burns. If the steam generator is automatically flooded during sterilization, the total steam pressure will drop rapidly. If necessary, open the steam valve appropriately to maintain pressure, but carefully observe pressure changes. As steam passes through the tank, the pressure in the tank drops rapidly after sterilization. Note the chance of passing through sterile air. Try not to have the air compressor and steam generator working at the same time. During the fermentation tank sterilization process, the air filter pressure should not exceed 0.2MP. The size of condensate will affect the concentration of the liquid in the tank after sterilization, so the increase of steam condensate should be considered.